Yes I did some testing for this. Running sprayer software (Patriot) in a tractor you can set an A-B guidence line and use the onscreen lightbar but because you cannot run the "tractor" software at the same time the autosteer will not engage. This is because each machine type has different steering sensor and override switch types. A 2wd tractor uses a potentiometer which has a voltage output range opposite of the yaw type sensor used on the Patriot. So durring a left turn the poteniometer voltage moves opposite the way a yaw sensor voltage output moves. If you try to run in the tractor it will stall the steering, when it needs to move left it will move slightly then stop because the steering sensor is telling it that it is instead making a right turn. Also you have to splice into the operator presence seat wire and run it into the Nav2 connector because sprayer software looks for the operator presence to come from the NAV while tractor software expects it to come from the ICU.
In theory if you reversed the sterring poteniometer wires on a 2wd tractor then autosteer would work. I have not tested that .